Glucose Oxidase

Recombinant, Diagnostic Reagent Grade

 

From yeast cells with cloned gene encoding genetically engineered fungus Aspergillus niger glucose oxidase.

Catalog Number: GPE0008
CAS No.: 9001-37-0 E.C.:1.1.3.4
Synonyms:  β-D-Glucose: oxygen 1- oxidoreductase, G.Od., GOx

 

Properties

pH range

 4-7

optimum pH: 4.5

Molecular Weight

Approx.160 KDa

Storage Temperature

-20 °C recommended for lyophilized powder

Forms

The product is available as lyophilized powder and also as buffered aqueous glycerol solution

Temperature profile

Optimum temperature 42°C

Temperature range of enzyme activity: 40-50°C

Specific activity

≥ 45 U/mg lyophilized powder

Unit definition:

One unit will oxidize 1.0 μmole of β-D-glucose to D-gluconolactone and H2O2 per min at pH 5.1 at 35 °C, equivalent to an O2 uptake of 22.4 μl per min. If the reaction mixture is saturated with oxygen, the activity may increase by up to 100%.

Activity

≥ 45 U/mg lyophilized powder

Purity and Quality

Reagent Grade

Diagnostic Reagent Grade(superior purity)

May contain traces of amylase, maltase, glycogenase, invertase, and galactose oxidase

Product Description

General Description:

Glucose oxidase is a well-characterised flavoprotein that catalyses oxidation of β-D-Glucose at its first hydroxyl group, utilizing molecular oxygen as the electron acceptor, to produce D-glucono-delta-lactone and hydrogen peroxide. Glucose oxidase is a homodimeric enzyme, with an FAD molecule moncovalently but tightly bound at the active site of each 80 KDa subunit. The enzyme consists of two identical polypeptide chain subunits (80,000 Daltons) covalently linked by disulfide bonds. Each subunit contains one mole of Fe and one mole of FAD (flavin-adenine dinucleotide). The molecule consists of approximately 74% protein, 16% neutral sugar and 2% amino sugars. The FAD is replaceable with FHD (flavin-hypoxanthine dinucleotide) without loss of activity.

Application:

Diagnostic application: Glucose oxidase can be utilized in the enzymatic determination of D-glucose in solution. As glucose oxidase oxidizes β-D-glucose to D-gluconolactate and hydrogen peroxide, horseradish peroxidase is often used as the coupling enzyme to visualize colorimetrically the formed hydrogen peroxide for the rapid determination of free glucose in samples.

Enzymatic glucose biosensors use an electrode instead of O2 to take up the electrons needed to oxidize glucose and produce an electronic current in proportion to glucose concentration, which is the technology behind the disposable glucose sensor strips used by diabetics to monitor serum glucose levels.

Food industrial application: Glucose oxidase assay allows the monitoring of glucose levels in fermentation and bioreactors. It also helps food preservation by removing oxygen from food, wine, beer packaging and bottling.  Glucose oxidase is widely used to remove residual glucose to inhibit non-enzymatic browning.

Advantages

An important characteristic of A.niger-derived GOD is its very high substrate specificity for glucose, which makes it perfectly fits in enzyme’s application for measuring glucose in the presence of other sugars.

Recombinant production of glucose oxidase has resulted in lower price/unit charges of enzyme products that allows our customers to experience substantial cuts in cost.

 

Preparation Notes

Activators and Inhibitors

Glucose oxidase does not require any activator, but it is inhibited by Ag+, Hg2+, Cu2+, phenylmercuric acetate, and p-chloromercuribenzoate (pCMB). It is not inhibited by the nonmetallic SH reagents: N-ethylmaleimide, iodoacetate, and iodoacetamide.

Stability

Lyophilized powder is stable for 3 years when stored below 4°C.

Buffered aqueous glycerol solution is stable for 1 years when stored at -20°C.


Transportation and Storage:

Buffered aqueous glycerol solution: transport with blue ice (below 4°C ). Storage Recommended at -20°C

Lyophilized powder: transport at room temperature. Storage Recommended below 4°C.

Storage buffer: 20 mM Tris-Cl (pH 8.0), 2 mM MgCl2, 20 mM NaCl, 50% glycerol.

Dilution buffer: 20 mM Tris-Cl (pH 8.0), 2 mM MgCl2, 20 mM NaCl.

Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

 

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