| Glucose Dehydrogenase (FAD-dependent ) Recombinant, Molecular Biology Grade Source: | |
| Catalog Number: GPE006 | |
| CAS No.:9035-82-9 | E.C.: 1.1.5.9 |
| Synonyms: FAD dependent glucose dehydrogenase, FAD glucose dehydrogenase, FAD-dependent GDH, flavin adenine dinucleotide-dependent glucose dehydrogenase | |
| Properties | |
| Molecular mass | ≥65 kDa |
| pI | 5.6 |
| pH range | |
| optimum pH range | |
| Storage Temperature | -20 °C Recommended |
| Form | lyophilized powder |
| Temperature profile | |
| Specific activity | ≥300 U/mg |
| Unit Definition: One unit of Glucose Dehydrogenase (FAD-dependent) activity is defined as the amount of enzyme that will convert 1 µmole of glucose to gluconic acid and hydrogen peroxide per minute at pH 6.5 at 37°C. | |
| Purity and Quality | |
| ≥95%（SDS-PAGE） Quality Control: The Glucose Dehydrogenase (FAD-dependent) Lyophilized Powder is tested for its activity, purity, and absence of contaminating nucleases, proteases, and endotoxins. The activity of the enzyme is measured using a spectrophotometric assay based on the production of hydrogen peroxide. The purity of the enzyme is assessed by SDS-PAGE and western blotting using specific antibodies. The absence of contaminating nucleases, proteases, and endotoxins is confirmed by gel electrophoresis and Limulus amoebocyte lysate (LAL) assay. | |
| Product Description | |
| General Description: Glucose Dehydrogenase (FAD-dependent) is an enzyme that catalyzes the oxidation of glucose to produce gluconic acid and hydrogen peroxide. This enzyme requires FAD (flavin adenine dinucleotide) as a cofactor to function. This lyophilized powder is a highly purified form of Glucose Dehydrogenase (FAD-dependent) that has been produced using recombinant DNA technology. The Glucose Dehydrogenase (GDH) is highly specific for the conversion of β-D-glucose and has low efficiency towards other monosaccharides. It is a fast and thermally stable enzyme that does not produce precipitation or turbidity after completion of the reaction. Application: Glucose dehydrogenase (GDH) has a wide range of applications in various fields including biotechnology, biochemistry, and medicine. Some of the key applications are: 1. Biosensors: GDH is widely used in the development of glucose biosensors for monitoring blood glucose levels in diabetes patients. 2. Biofuels: GDH can be used to produce biofuels such as ethanol and butanol from renewable resources like sugar, corn, and cellulosic materials. 3. Food and Beverage Industry: GDH is used as a biocatalyst in the production of high-fructose corn syrup, which is widely used as a sweetener in the food and beverage industry. 4. Pharmaceuticals: GDH can be used in the synthesis of certain pharmaceuticals, such as ascorbic acid and D-gluconic acid. 5. Bioremediation: GDH has been found to play a role in the bioremediation of environmental pollutants such as heavy metals and organic pollutants. 6. Biotechnology: GDH has potential applications in the biotechnology industry for the production of chiral chemicals and fine chemicals. | |
| Preparation Notes | |
| Dilution buffer: 10 mM Tris（pH 8.0）， 5 mM NaCl Storage buffer: 10 mM Tris（pH 8.0），5 mM NaCl | |
| Inhibitors | |
| Storage | The Glucose Dehydrogenase (FAD-dependent) Lyophilized Powder should be stored at -20°C or lower in a non-frost-free freezer. It is recommended to avoid repeated freeze-thaw cycles to preserve the activity of the enzyme. |
| Stability/Shelf life： The product is stable for at least 12 months from the date of shipment when stored properly. | |
| Precautions and Disclaimer | |
| Safety Information: The Glucose Dehydrogenase (FAD-dependent) Lyophilized Powder is not hazardous, but standard laboratory safety procedures should be followed when handling this product. Avoid contact with skin and eyes. In case of accidental contact, rinse with plenty of water and seek medical attention if necessary. This product is for R&D use only, not for drug, household, or other uses. | |
