Recombinant Binuclease

Binuclease, A Broad-spectrum nucleases, Benzonase equivalent Recombinant, Molecular Biology Grade From yeast cells with cloned gene encoding genetically engineered Serratia marcescens endonuclease
Catalog Number: GPE004001
CAS No.: 9025-65-4	E.C.: 3.1.30.2
Synonyms: Serratia marcescens Binuclease; unrestricted endonuclease

Properties
Molecular Weight	27.9 kD
pH range	pH 6~10
Optimum pH 8.0
Storage Temperature	-20 °C for lyophilized powder
Form	The product is available as lyophilized powder and also as buffered aqueous glycerol solution
Temperature profile	Optimum temperature 37°C
Temperature range of enzyme activity: 0~42°C
Specific Activity	≥ 1000 kU/mg protein
Unit definition: According to the protocol from Sigma-Aldrich, one unit of Binuclease will digest sonicated salmon sperm DNA to produce acid-soluble oligonucleotides equivalent to a ΔA260nm of 1.0 in 30 minutes at pH 8.0 at 37°C. Activity ≥ 20 kU/mg lyophilized powder ≥ 250 U/μL buffered aqueous glycerol solution

Purity and Quality
Superior purity: ≥90%; (SDS-PAGE) Protease: None Detected Endotoxin: ＜0.25EU/KU Tachypleus Amebocyte Lysate Test Bioburden: : Microorganisms count ≤5CFU/g
Product Description
General Description: Binuclease is a genetically engineered endonuclease from Serratia marcescens gene that can digest all forms of DNA and RNA, including single stranded, double stranded, linear, circular and supercoiled DNA and RNA. The enzyme cleaves the phosphodiester bond of nucleic acids, producing 5’ monophosphate terminated oligonucleotides 2-5 bases in length. Binuclease is not a sequence dependent nuclease, capable of cleaving the phosphodiester. Advantages Binuclease is produced from eukaryotic yeast cells that avoid the contamination of endotoxin from prokaryotic cells. Binuclease is highly stable under harsh industrial conditions and very cost effective, which make it a perfect alternative for Benzonase® endonuclease and other equivalents. The specific activity of Binuclease was measured at ≥ 1000 kU/mg protein, which is much higher than any existing products (as much as 200%). Application: Gproan Binuclease Endonuclease cleaves all forms of DNA and RNA, which makes it an ideal tool to eliminate nucleic acids (DNA and RNA) contamination in vaccine, antibody, cell therapy and other biological products for therapeutic use to meet FDA guidelines that nucleic acid contamination should be limited to 100 pg. per dose in the end product. Binuclease reduces nucleic acid load and viscosity in cell lysates during purification to facilitate the filtration/ultrafiltration of solutions, reduce processing time, improve the separation of pellets and supernatants in centrifugations and increase the efficiency of chromatographic purification, resulting significant improvements on both yield and purity of cell-derived particles such as viruses, AAV vectors or inclusion bodies. Bioanalytical applications: Sample preparation for ELISA, chromatography or two-dimensional electrophoresis (protein mapping), and footprint analysis to get improved resolution and increased recovery of samples.
Preparation Notes
Dilution buffer: 20 mM Tris-Cl (pH 8.0), 2 mM MgCl₂, 20 mM NaCl. Storage buffer: 20 mM Tris-Cl (pH 8.0), 2 mM MgCl₂, 20 mM NaCl, 50% glycerol.
Activators	1~10 mM Mg²⁺
Inhibitors
The following common reagents have inhibitory effects on the activity of broad-spectrum nucleases. Within the concentration ranges listed below, the loss of broad-spectrum nuclease activity is within 50%. Urea: 0-2M SDS: 0-0.05% KCl: 0-0.2M Guanidine Hydrochloride: 0-2M NaCl: 0-0.15M EDTA: 0-1M Ammonium Sulfate: 0-100mM Triton concentrations below 1.5% have no significant effect on Binuclease activity. The activity of Binuclease is influenced by ion concentration, reaction temperature, and pH. It is recommended to determine the optimal concentration during initial use.
Stability
Shelf life of Lyophilized powder: 3 years when stored below 4°C. Shelf life of Buffered aqueous glycerol solution: 1 years when stored at -20°C.
Handling and Storage: Buffered aqueous glycerol solution: Shipped with blue ice. Storage Recommended at -20°C Lyophilized powder: Shipped at room temperature. Storage Recommended at 4°C.
Precautions and Disclaimer
This product is for R&D use only, not for drug, household, or other uses.